{"id":6866795069553,"title":"BotuCrio Freezing Extender, For Equine, 25ml, Each","handle":"botucrio-freezing-extender-for-equine-25ml-each","description":"\u003cul\u003e\n\u003cli\u003eBotuCrio is an innovative freezing extender developed for stallion sperm cryopreservation. The formula was designed after long years of research, fertility trials, and field tests. The extender aims to protect the stallion sperm from cryodamage and maintain fertility post-thawing.\u003c\/li\u003e\n\u003cli\u003eScientific papers have demonstrated excellent results using BotuCrio, since the extender provided higher motility parameters, preserved the sperm membrane integrity, and contributed to better fertility rates.\u003c\/li\u003e\n\u003cli\u003eBotuCrio is the worldwide extender most used for stallion sperm cryopreservation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eHow to use\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAfter centrifugation, discard the supernatant and add BotuCrio directly to the sperm pellet at a concentration of 200 million sperm per mL. Pack and seal the straws.\u003c\/li\u003e\n\u003cli\u003eFor the conventional freezing method, stabilize the straws at 5oC for 20 minutes.\u003c\/li\u003e\n\u003cli\u003eThen, place the straws in nitrogen vapor (Styrofoam box), 3 to 6 cm above liquid nitrogen level for 20 minutes.\u003c\/li\u003e\n\u003cli\u003eFor thawing, the straws must be placed in a water bath at 37oC for 30 seconds or 46oC for 20 seconds.\u003c\/li\u003e\n\u003cli\u003eStore it frozen at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days. Add the contents of the bottle directly into the centrifuged semen.\u003c\/li\u003e\n\u003c\/ul\u003e","published_at":"2023-08-30T09:21:30-05:00","created_at":"2023-06-19T14:03:18-05:00","vendor":"Botupharma","type":"","tags":["AI Kits","Artificial Insemination (AI)","Chilled Transport","Cryogenic Storage \u0026 Shipping","Extenders \u0026 Additives","Filling - Labeling \u0026 Freezing Equipment","Horse","Non-Stocked","Semen Collection - Extenders - Packaging - Freezing - Shipping"],"price":6789,"price_min":6789,"price_max":6789,"available":true,"price_varies":false,"compare_at_price":null,"compare_at_price_min":0,"compare_at_price_max":0,"compare_at_price_varies":false,"variants":[{"id":40207961096305,"title":"Default Title","option1":"Default Title","option2":null,"option3":null,"sku":"BOTU0006","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"BotuCrio Freezing Extender, For Equine, 25ml, Each","public_title":null,"options":["Default Title"],"price":6789,"weight":227,"compare_at_price":null,"inventory_management":"shopify","barcode":"","quantity_rule":{"min":1,"max":null,"increment":1}}],"images":["\/\/store.agtechinc.com\/cdn\/shop\/files\/Screenshot2023-06-05at15-04-22BotuCRIO-BotupharmaUSA.png?v=1687201419"],"featured_image":"\/\/store.agtechinc.com\/cdn\/shop\/files\/Screenshot2023-06-05at15-04-22BotuCRIO-BotupharmaUSA.png?v=1687201419","options":["Title"],"media":[{"alt":null,"id":23181203013745,"position":1,"preview_image":{"aspect_ratio":0.589,"height":401,"width":236,"src":"\/\/store.agtechinc.com\/cdn\/shop\/files\/Screenshot2023-06-05at15-04-22BotuCRIO-BotupharmaUSA.png?v=1687201419"},"aspect_ratio":0.589,"height":401,"media_type":"image","src":"\/\/store.agtechinc.com\/cdn\/shop\/files\/Screenshot2023-06-05at15-04-22BotuCRIO-BotupharmaUSA.png?v=1687201419","width":236}],"content":"\u003cul\u003e\n\u003cli\u003eBotuCrio is an innovative freezing extender developed for stallion sperm cryopreservation. The formula was designed after long years of research, fertility trials, and field tests. The extender aims to protect the stallion sperm from cryodamage and maintain fertility post-thawing.\u003c\/li\u003e\n\u003cli\u003eScientific papers have demonstrated excellent results using BotuCrio, since the extender provided higher motility parameters, preserved the sperm membrane integrity, and contributed to better fertility rates.\u003c\/li\u003e\n\u003cli\u003eBotuCrio is the worldwide extender most used for stallion sperm cryopreservation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eHow to use\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAfter centrifugation, discard the supernatant and add BotuCrio directly to the sperm pellet at a concentration of 200 million sperm per mL. Pack and seal the straws.\u003c\/li\u003e\n\u003cli\u003eFor the conventional freezing method, stabilize the straws at 5oC for 20 minutes.\u003c\/li\u003e\n\u003cli\u003eThen, place the straws in nitrogen vapor (Styrofoam box), 3 to 6 cm above liquid nitrogen level for 20 minutes.\u003c\/li\u003e\n\u003cli\u003eFor thawing, the straws must be placed in a water bath at 37oC for 30 seconds or 46oC for 20 seconds.\u003c\/li\u003e\n\u003cli\u003eStore it frozen at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days. Add the contents of the bottle directly into the centrifuged semen.\u003c\/li\u003e\n\u003c\/ul\u003e"}

BotuCrio Freezing Extender, For Equine, 25ml, Each

Item #/Sku: BOTU0006
Manufacturer: Botupharma

Harmonized Shipping Code: 3821.00.0010

This product is NOT STOCKED in our warehouse. However, you CAN still order it online. It will take up to 7 business days to arrive at our warehouse. Please consider the following :

Agtech ships all orders complete. We will not ship your order until ALL products in your order have arrived at our warehouse and passed quality control. If you decide to order both stocked and non-stocked items in a single transaction (same shopping cart), Agtech will honor the UPS shipping speed you choose AFTER all products have arrived at our warehouse.

If you cannot wait for the non-stocked items to arrive, and need your regular, stocked items quickly...simply remove the non-stocked items from your shopping cart and checkout. You can then create a new shopping cart with just non-stocked items. All non-stocked items will have this yellow notice.

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  • BotuCrio is an innovative freezing extender developed for stallion sperm cryopreservation. The formula was designed after long years of research, fertility trials, and field tests. The extender aims to protect the stallion sperm from cryodamage and maintain fertility post-thawing.
  • Scientific papers have demonstrated excellent results using BotuCrio, since the extender provided higher motility parameters, preserved the sperm membrane integrity, and contributed to better fertility rates.
  • BotuCrio is the worldwide extender most used for stallion sperm cryopreservation.

How to use

  • After centrifugation, discard the supernatant and add BotuCrio directly to the sperm pellet at a concentration of 200 million sperm per mL. Pack and seal the straws.
  • For the conventional freezing method, stabilize the straws at 5oC for 20 minutes.
  • Then, place the straws in nitrogen vapor (Styrofoam box), 3 to 6 cm above liquid nitrogen level for 20 minutes.
  • For thawing, the straws must be placed in a water bath at 37oC for 30 seconds or 46oC for 20 seconds.
  • Store it frozen at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days. Add the contents of the bottle directly into the centrifuged semen.

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